Journal: International Journal of Nanomedicine

Article Title: Preparation of Budesonide-Loaded Liposomal Nanoparticles for Pulmonary Delivery and Their Therapeutic Effect in OVA-Induced Asthma in Mice

doi: 10.2147/ijn.s441345

Figure Lengend Snippet: Figure 7 Therapeutic effect of BUD-LNPs on OVA-induced asthma in mice. (a) Images of respiratory bronchioles and alveoli stained with AB-PAS after undergoing various treatments. Scale bar: 50 μm. (b) H&E staining images of respiratory bronchioles and alveoli after different treatments. Scale bar: 50 μm. The results of ELISA about protein levels of cytokines (c) IL-4, (d) IL-5, (e) IL-13 of supernatant from BALF. Data were presented as mean ± S.D. (n = 6) *P < 0.05; **P < 0.01; ***P < 0.001.

Article Snippet: Materials and Agents DOTAP (AVT, China, 132172-61-3), DOPE (Macklin, China, 4004-05-1), CHOL (Macklin, China, 57-88-5), DSPEPEG2000 (Macklin, China, 147867-65-0), rhodamine B (Macklin, China, 81-88-9), budesonide (Aladdin, China, 5137229-3), chloroform and methanol (Beijing Chemical Factory, China), OVA (Sigma-Aldrich, USA, 9006-59-1), Al(OH)3 (Sigma-Aldrich, USA, 77161), Hoechst 33342 (Sigma-Aldrich, USA, 1853314), Cy5-NHS (Meilun Biotechnology, China, 146368-14-1), cell counting Kit-8 (Dojindo, Japan, CK04), mouse IL-4 ELISA kits (Liankebio, China, EK204), mouse IL-5 ELISA kits (Liankebio, China, EK205), mouse IL-13 ELISA kits (Liankebio, China, EK213), AST assay kits (Nanjing Jiancheng, China, C010-2-1), ALT assay kits (Nanjing Jiancheng, China, C009-2-1), AKP assay kits (Nanjing Jiancheng, China, A059-2-2), BUN assay kits (Nanjing Jiancheng, China, C013-2-1), CRE assay kits (Nanjing Jiancheng, China, C011-2-1), UA assay kits (Nanjing Jiancheng, China, C012-2-1). https://doi.org/10.2147/IJN.S441345 DovePress International Journal of Nanomedicine 2024:19 674 Powered by TCPDF (www.tcpdf.org) Cell Culture RAW264.7 cells and the corresponding complete medium were obtained from Zhong Qiao Xin Zhou Biotechnology Co., Ltd. (Shanghai, China).

Techniques: Staining, Enzyme-linked Immunosorbent Assay

Journal: Molecular Medicine Reports

Article Title: Modified Pulsatilla decoction attenuates oxazolone-induced colitis in mice through suppression of inflammation and epithelial barrier disruption

doi: 10.3892/mmr.2016.5358

Figure Lengend Snippet: Modified Pulsatilla decoction treatment significantly reduces the secretion of pro-inflammatory cytokines. (A) mRNA expression levels of IL-5 and IL-13 in colon tissues from oxazolone-induced colitis mice, following treatment with or without modified Pulsatilla decoction or SASP, were determined using reverse transcription-quantitative polymerase chain reaction analysis. (B) Protein levels of IL-5 and IL-13 in colon tissues from oxazolone-induced colitis mice, following treatment with or without modified Pulsatilla decoction or SASP, were determined using ELISA. Data are expressed as the mean ± standard deviation and are representative of three independent experiments. * P<0.05, ** P<0.01 vs. the control group. PBS, phosphate-buffered saline; SASP, sulfasalazine; IL, interleukin.

Article Snippet: The protein levels of IL-5 and IL-13 in the supernatants of the colon tissue lysates were measured using Mouse IL-13 Quantikine ELISA kit and Mouse IL-5 Quantikine ELISA kit (R&D Systems, Minneapolis, MN, USA), according to the manufacturer's protocol.

Techniques: Modification, Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Standard Deviation, Control, Saline